The biopolymer films have a low thermal stability, poor gas barrier properties, low tensile strength, and low elasticity despite being environmentally friendly and biodegradable. A composite film made of clay and agarose biopolymer was developed to boost its heat stability. The film was then given glycerol to improve its flexibility. Solvent casting, clay with a weight percentage of 3 or 5, and clay that had been treated with agarose and amino glycoside medications were all used to create the films. The clay and modified clay agarose films were also characterised using thermal analysis, PXRD, and AFM methods. Two gram-positive (Staphylococcus aurous, Bacillus subtilis) and two gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria were used to test the composite films' efficacy in treating wounds.