Chlorophyll is an antioxidant which usually found in nature especially in green leafy plants. The chlorophyll content of eight mulberry varieties such as AR12,S13, S146, S1635 andTR10 and their effects on silkworm growth were studied. Total chlorophyll of all the varieties was extracted individually using acetone as solvent and detected using spectrophotometer at two wavelengths (645 nm and 663 nm). The maximum total chlorophyll content was observed in S146. The bottom leaves of S146 showed highest chlorophyll content (21.115 mg/g) compared to apical (13.338 mg/g) and middle (20.567 mg/g) leaves. The diseased leaves of S146 showed least quantity of chlorophyll content (12. 342 mg/g). The silkworm fed with S146 mulberry leaves showed better rearing performance than the worms fed by leaves of other varieties. S146 fed larvae showed better cocoon performance effective rate of rearing (ERR %), cocoon weight, pupa weight, shell weight and filamentlength compared to other mulberry varieties. It shows the effect of chlorophyll content on rearing performance of B.mori.