To scientifically and reasonably analyze the bending vibration frequency of the new type composite box girder bridge with corrugated steel webs, taking into account the shear deformation effect of corrugated steel webs and the shear lag effect of box girder, the Galerkin method and the Hamilton principle were used to deduce the free vibration control differential equation and natural boundary conditions of the bridge type. According to the natural boundary conditions, the calculation formula of the bending vibration frequency of the new type composite box girder bridge with corrugated steel webs under the influence of shear deformation effect and shear lag effect was solved. The results were compared with the measured results and ANSYS finite element results, and the influencing factors of the bending vibration frequency were analyzed. The results showed that the calculation results of the bending vibration frequency were in good agreement with the measured results and the ANSYS finite element results, which verified the correctness of the derived frequency calculation formula. The bending vibration frequency increased with the increase of the high-span ratio. When it was less than 0.05, the increase of the bending vibration frequency was relatively gentle. When the height-span ratio was greater than 0.05, the increase of the bending vibration frequency was more significant. The bending vibration frequency increased with the increase of the width-span ratio, but the overall increase was not obvious. The frequency increased with the increase of the thickness of the corrugated steel web, and the higher the order, the more significant the increase. The shear lag effect of the box girder had little effect on the bending vibration frequency, and the maximum error of the first 5 orders was only 6.73%. The shear deformation of the corrugated steel webs had a great influence on the bending vibration frequency, and the maximum error of the first five orders was as high as 51.18%