ANTIMICROBIAL ASSESSMENT OF COPOLYMER DERIVED FROM 2-AMINOTHIOPHENOL, ETHYLENEDIAMINE AND FORMALDEHYDE

Abstract

Copolymer (2-ATEAF) was prepared by condensing the 2-aminothiophenol, ethylenediamine, and formaldehyde in the presence of hydrochloric acid. To analyse the elemental composition of the copolymer, the CHNS analysis was conducted, and the number-average molecular weight (Mn) and polydispersity index were precisely determined through gel permeation chromatography (GPC) analysis, utilizing either dimethylformamide (DMF) or tetrahydrofuran (THF) as the mobile phase and employing polystyrene standards for accurate calibration. The use of the Huggins and Kraemer equations, intrinsic viscosity, and viscometric constants (k and a) were determined in dimethyl sulfoxide (DMSO). The structure of the copolymer was explained by a range of spectroscopy techniques including UV–Visible spectroscopy, FTIR spectroscopy, and ¹H NMR spectroscopy were employed for characterization. Besides the SEM and the powder x-ray diffraction (PXRD), it was also established that the material was amorphous using scanning electron microscopy (SEM). Antimicrobial assay was done by using aspergillus niger, staphylococcus aureus and bacillus subtilis through agar well diffusion. Gram-positive species of bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-negative species of bacteria ( Escherichia coli ) were also tested. Ethylenediamine moiety was evaluated as a factor of antimicrobial and antifungal effect.