The green synthesis of zinc oxide (ZnO) nanoparticles with the help of a natural reducing and stabilizing agent Caralluma attenuata extract. The environmentally friendly synthesis method does not require the use of dangerous chemicals and increases biocompatibility. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-Visible spectroscopy, Raman spectroscopy, and scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX) were used to characterize the synthesized nanoparticles. The results of XRD indicated a predominantly hexagonal structure of wurtzite with multiphase tendencies. FTIR analysis indicated the presence of functional groups of hydroxyl and Zn-O bonds, which confirmed the formation of nanoparticles. The UV- Vis analysis revealed that it had a good absorption at the ultraviolet region implying that it had a broad bandgap which could be used in optoelectronics. SEM demonstrated agglomerated nanoscale particles, and elemental composition was verified by EDX. Dielectric experiments revealed that the dielectric constant and loss decreased with frequency, which is appropriate in nano-electronic devices. The agar well diffusion method was used to evaluate antifungal activity against Aspergillus fumigatus, Candida tropicalis, and Trichophyton rubrum. The findings indicated a high degree of inhibition with the greatest activity being against Aspergillus fumigatus. Reactive oxygen species production and nanoparticle-cell interactions are credited with the increased antifungal activity. Given the above, the research paper has shown the potential of ZnO nanoparticles mediated by Caralluma attenuata in biomedical and nano-electronic applications..